Deformation, crimp, and alignment patterns of collagen fibers in soft tissues have been measured using a novel testing system combining controlled loading with synchronized high-resolution imaging. Marker-less feature tracking was used to measure 2-D local deformations, and a radon transform was used to compute quantitative maps of crimp patterns and local tissue fiber orientation. We found highly nonuniform deformation and crimp patterns, with the highest strains near the clamps, and the lowest strains in the midsubstance region.

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