It has long been thought that an optical sensor, such as a light waveguide implemented total analysis system (TAS), is one of the functional components that will be needed to realize a “ubiquitous human healthcare system” in the near future. We have already proposed the fundamental structure for a light waveguide capable of illuminating a living cell or particle running along a microfluidic channel, as well as of detecting fluorescence even from the extremely weak power of such a minute particle. In order to develop novel functions to detect the internal structure of living cells conveniently, an angular scanning method that sequentially changes the direction of illumination of the minute cell or particle may be crucial. In this research paper, we investigated fluorescence detection from moving resin particles by switching direction of laser power illumination by radially arranged outlet portions of several light waveguides, as a step-by-step examination toward this novel scanning method. To cost effectively construct an experimental system able to incorporate a mechanism of switching illumination direction, we utilized a monolithic resin-based TAS chip with plural waveguide pairs whose outlet portions were arranged radially and inlet portions were arranged in parallel, and a forced vibration mechanism on an optical fiber tip by a piezoelectric actuator. With this chip and system, we constructed an experimental system to detect extremely weak fluorescence using micro particles with a fluorescent substance attached and an optical TAS chip that incorporated a microfluidic channel and five pairs of laser-power-delivering light waveguide.
- Information Storage and Processing Systems Division
Evaluation of Fluorescence Emitting Characteristics of a Micro Particle by Illumination Angle Scanning Utilizing a Resin-Based Monolithic TAS Chip
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Ohkubo, T, Terada, N, & Yoshida, Y. "Evaluation of Fluorescence Emitting Characteristics of a Micro Particle by Illumination Angle Scanning Utilizing a Resin-Based Monolithic TAS Chip." Proceedings of the ASME 2016 Conference on Information Storage and Processing Systems. ASME 2016 Conference on Information Storage and Processing Systems. Santa Clara, California, USA. June 20–21, 2016. V001T06A002. ASME. https://doi.org/10.1115/ISPS2016-9529
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