Ultra-fast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultra-fast cooling rate. Experimental results show that the average cooling rate of dimenthylsulphoxide cryoprotective solution reaches 150,000°C/min in a temperature range from 10°C to −180°C. The ultra-fast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.
- Heat Transfer Division
An Ultra Fast Cooling Method for Cell Vitrification Cryopreservation Utilizing Thin Film Evaporation
- Views Icon Views
- Share Icon Share
- Search Site
Su, F, Zhao, N, Deng, Y, Tian, B, Mu, C, & Ma, H. "An Ultra Fast Cooling Method for Cell Vitrification Cryopreservation Utilizing Thin Film Evaporation." Proceedings of the ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer. Volume 2: Micro/Nano-Thermal Manufacturing and Materials Processing; Boiling, Quenching and Condensation Heat Transfer on Engineered Surfaces; Computational Methods in Micro/Nanoscale Transport; Heat and Mass Transfer in Small Scale; Micro/Miniature Multi-Phase Devices; Biomedical Applications of Micro/Nanoscale Transport; Measurement Techniques and Thermophysical Properties in Micro/Nanoscale; Posters. Biopolis, Singapore. January 4–6, 2016. V002T13A001. ASME. https://doi.org/10.1115/MNHMT2016-6319
Download citation file: