Peptide modification of hydrogel-forming materials is being widely explored as a means to regulate the phenotype of cells immobilized within the gels. Alternatively, we hypothesized that the adhesive interactions between cells and peptides coupled to the gel-forming materials would also enhance the overall mechanical properties of the gels. To test this hypothesis, alginate polymers were modified with RGDSP-containing peptides and the resultant polymer was used to encapsulate C2C12 myoblasts. The mechanical properties of these gels were then assessed as a function of both peptide and cell density using compression and tensile tests. Overall, it was found that above a critical peptide and cell density, encapsulated myoblasts were able to provide additional mechanical integrity to hydrogels composed of peptide-modified alginate. This occurred presumably by means of cell-peptide cross-linking of the alginate polymers, in addition to the usual cross-linking. These results are potentially applicable to other polymer systems and important for a range of tissue engineering applications.
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e-mail: mooneyd@deas.harvard.edu
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April 2005
Technical Papers
Cellular Cross-linking of Peptide Modified Hydrogels
Jeanie L. Drury,
Jeanie L. Drury
Departments of Biologic and Materials Science, Biomedical Engineering, and Chemical Engineering, University of Michigan
, Ann Arbor, MI
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Tanyarut Boontheekul,
Tanyarut Boontheekul
Department of Chemical Engineering, University of Michigan
, Ann Arbor, MI
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David J. Mooney
e-mail: mooneyd@deas.harvard.edu
David J. Mooney
Departments of Biologic and Materials Science, Biomedical Engineering, and Chemical Engineering, University of Michigan
, Ann Arbor, MI
Search for other works by this author on:
Jeanie L. Drury
Departments of Biologic and Materials Science, Biomedical Engineering, and Chemical Engineering, University of Michigan
, Ann Arbor, MI
Tanyarut Boontheekul
Department of Chemical Engineering, University of Michigan
, Ann Arbor, MI
David J. Mooney
Departments of Biologic and Materials Science, Biomedical Engineering, and Chemical Engineering, University of Michigan
, Ann Arbor, MIe-mail: mooneyd@deas.harvard.edu
J Biomech Eng. Apr 2005, 127(2): 220-228 (9 pages)
Published Online: November 18, 2004
Article history
Received:
November 14, 2003
Revised:
October 17, 2004
Accepted:
November 18, 2004
Citation
Drury, J. L., Boontheekul, T., and Mooney, D. J. (November 18, 2004). "Cellular Cross-linking of Peptide Modified Hydrogels." ASME. J Biomech Eng. April 2005; 127(2): 220–228. https://doi.org/10.1115/1.1865194
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