Abstract
The lack of sufficient mechanical stimulation to the human bone, results in disuse osteoporosis. Low-intensity pulsed ultrasound (LIPUS) promotes fracture healing and the treatment of disuse osteoporosis, but its biomechanical mechanism remains unknown. Simulative research on the mechanical effects of LIPUS on disuse trabecular bone and osteoblasts have been performed. The von Mises stress of disuse trabecular bone and osteoblasts obviously increased under LIPUS irradiation. The average von Mises stress of osteoblasts were two orders of magnitude higher under the irradiation of simulant LIPUS than that without LIPUS irradiation, and the von Mises stress of osteoblasts was positively correlated with the amplitude of sound pressure excitation. The results showed that LIPUS irradiation could obviously improve the mechanical micro-environment of trabecular bone and osteoblasts to alleviate the lack of mechanical stimulation. The results of the research can reveal the biomechanical mechanism of LIPUS in the treatment of disuse osteoporosis to some extent and provide theoretical guidance for clinical treatment of disuse osteoporosis through physical methods.
Issue Section:
Research Papers
References
1.
Kameo
,
Y.
,
Sakano
,
N.
, and
Adachi
,
T.
, 2020
, “
Theoretical Concept of Cortical to Cancellous Bone Transformation
,” Bone Rep.
,
12
, p. 100260
.10.1016/j.bonr.2020.1002602.
Pei
,
B. Q.
,
Wang
,
T. M.
, and
Wang
,
J. Q.
, 2008
, “
The Comprehensive Biomechanical Analysis of Microcosmic Trabecular Structure of Cancellous Bone
,” Beijing Biomed. Eng.
,
27
(2
), pp. 120
–122
(in Chinese).3.
Li
,
T. Y.
,
Chen
,
Z. B.
,
Gao
,
Y.
,
Zhu
,
L. S.
,
Yang
,
R. L.
,
Leng
,
H. J.
, and
Huo
,
B.
, 2020
, “
Fluid-Solid Coupling Numerical Simulation of Trabecular Bone Under Cyclic Loading in Different Directions
,” J. Biomech.
,
109
, p. 109912
.10.1016/j.jbiomech.2020.1099124.
Mullender
,
M. G.
, and
Huiskes
,
R.
, 1995
, “
Proposal for the Regulatory Mechanism of Wolff's Law
,” J. Orthop. Res.
,
13
(4
), pp. 503
–512
.10.1002/jor.11001304055.
Romano
,
C. L.
,
Romano
,
D.
, and
Logoluso
,
N.
, 2009
, “
Low-Intensity Pulsed Ultrasound for the Treatment of Bone Delayed Union or Nonunion: A Review
,” Ultrasound Med. Biol.
,
35
(4
), pp. 529
–536
.10.1016/j.ultrasmedbio.2008.09.0296.
Sun
,
S. X.
,
Tang
,
L.
,
Zhao
,
T. T.
,
Kang
,
Y. T.
,
Sun
,
L. J.
,
Liu
,
C. C.
,
Li
,
Y.
,
Xu
,
F.
,
Qin
,
Y. X.
, and
Ta
,
D.
, 2021
, “
Longitudinal Effects of Low-Intensity Pulsed Ultrasound on Osteoporosis and Osteoporotic Bone Defect in Ovariectomized Rats
,” Ultrasonics
,
113
, p. 106360
.10.1016/j.ultras.2021.1063607.
Du
,
J.
,
Li
,
S.
, and
Silberschmidt
,
V. V.
, 2020
, “
Trabecular Bone Remodelling: Finite-Element Simulation
,” Procedia Struct. Integr.
,
28
, pp. 577
–583
.10.1016/j.prostr.2020.10.0678.
Cao
,
Q.
,
Zhang
,
J. Y.
,
Liu
,
H. T.
,
Wu
,
Q.
,
Chen
,
J. C.
, and
Chen
,
G. Q.
, 2014
, “
The Mechanism of Anti-Osteoporosis Effects of 3-Hydroxybutyrate and Derivatives Under Simulated Microgravity
,” Biomaterials
,
35
(28
), pp. 8273
–8283
.10.1016/j.biomaterials.2014.06.0209.
Lin
,
X.
,
Xiao
,
Y. Y.
,
Zhang
,
K. W.
,
Yang
,
D.
,
Miao
,
Z. P.
,
Deng
,
X. N.
,
Chen
,
Z. H.
, and
Qian
,
A. R.
, 2022
, “
Knockdown of MACF1 Inhibits the Migration and Cytoskeletal Arrangement of Pre-Osteoclasts Induced by Simulated Microgravity
,” Acta Astronaut.
,
190
, pp. 149
–159
.10.1016/j.actaastro.2021.10.01010.
Cao
,
H. J.
,
Feng
,
L. F.
,
Wu
,
Z. X.
,
Hou
,
W. T.
,
Li
,
S. J.
,
Hao
,
Y. L.
, and
Wu
,
L.
, 2017
, “
Effect of Low-Intensity Pulsed Ultrasound on the Biological Behavior of Osteoblasts on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
,” Mater. Sci. Eng., C
,
80
, pp. 7
–17
.10.1016/j.msec.2017.05.07811.
Suzuki
,
N.
,
Hanmoto
,
T.
,
Yano
,
S.
,
Furusawa
,
Y.
,
Ikegame
,
M.
,
Tabuchi
,
Y.
,
Kondo
,
T.
,
Kitamura
,
K. I.
,
Endo
,
M.
,
Yamamoto
,
T.
,
Sekiguchi
,
T.
,
Urata
,
M.
,
Mikuni-Takagaki
,
Y.
, and
Hattori
,
A.
, 2016
, “
Low-Intensity Pulsed Ultrasound Induces Apoptosis in Osteoclasts: Fish Scales Are a Suitable Model for the Analysis of Bone Metabolism by Ultrasound
,” Comp. Biochem. Physiol., Part A: Mol. Integr. Physiol.
,
195
, pp. 26
–31
.10.1016/j.cbpa.2016.01.02212.
Kusuyama
,
J.
,
Bandow
,
K.
,
Shamoto
,
M.
,
Kakimoto
,
K.
,
Ohnishi
,
T.
, and
Matsuguchi
,
T.
, 2014
, “
Low Intensity Pulsed Ultrasound (LIPUS) Influences the Multilineage Differentiation of Mesenchymal Stem and Progenitor Cell Lines Through ROCK-Cot/Tpl2-MEK-ERK Signaling Pathway
,” J. Biol. Chem.
,
289
(15
), pp. 10330
–10344
.10.1074/jbc.M113.54638213.
Carvalho
,
D. C. L.
, and
Cliquet
,
A.
, 2004
, “
The Action of Low-Intensity Pulsed Ultrasound in Bones of Osteopenic Rats
,” Artif. Organs
,
28
(1
), pp. 114
–118
.10.1111/j.1525-1594.2004.07091.x14.
Wu
,
S. L.
,
Kawahara
,
Y.
,
Manabe
,
T.
,
Ogawa
,
K.
,
Matsumoto
,
M.
,
Sasaki
,
A.
, and
Yuge
,
L.
, 2009
, “
Low-Intensity Pulsed Ultrasound Accelerates Osteoblast Differentiation and Promotes Bone Formation in an Osteoporosis Rat Model
,” Pathobiology
,
76
(3
), pp. 99
–107
.10.1159/00020938715.
Pounder
,
N. M.
, and
Harrison
,
A. J.
, 2008
, “
Low Intensity Pulsed Ultrasound for Fracture Healing: A Review of the Clinical Evidence and the Associated Biological Mechanism of Action
,” Ultrasonics
,
48
(4
), pp. 330
–338
.10.1016/j.ultras.2008.02.00516.
Claes
,
L.
, and
Willie
,
B.
, 2007
, “
The Enhancement of Bone Regeneration by Ultrasound
,” Prog. Biophys. Mol. Biol.
,
93
(1–3
), pp. 384
–398
.10.1016/j.pbiomolbio.2006.07.02117.
Mansjur
,
K. Q.
,
Kuroda
,
S.
,
Izawa
,
T.
,
Maeda
,
Y.
,
Sato
,
M.
,
Watanabe
,
K.
,
Horiuchi
,
S.
, and
Tanaka
,
E.
, 2016
, “
The Effectiveness of Human Parathyroid Hormone and Low-Intensity Pulsed Ultrasound on the Fracture Healing in Osteoporotic Bones
,” Ann. Biomed. Eng.
,
44
(8
), pp. 2480
–2488
.10.1007/s10439-015-1533-y18.
Sun
,
L. J.
,
Sun
,
S. X.
,
Zhao
,
X. J.
,
Zhang
,
J.
,
Guo
,
J. Z.
,
Tang
,
L.
, and
Ta
,
D.
, 2019
, “
Inhibition of Myostatin Signal Pathway May Be Involved in Low-Intensity Pulsed Ultrasound Promoting Bone Healing
,” J. Med. Ultrason.
,
46
(4
), pp. 377
–388
.10.1007/s10396-019-00962-219.
Zhou
,
H. B.
,
Hou
,
Y. F.
,
Zhu
,
Z. M.
,
Xiao
,
W. X.
,
Xu
,
Q.
,
Li
,
L.
,
Li
,
X.
, and
Chen
,
W. C.
, 2016
, “
Effects of Low-Intensity Pulsed Ultrasound on Implant Osseointegration in Ovariectomized Rats
,” J. Ultrasound Med.
,
35
(4
), pp. 747
–754
.10.7863/ultra.15.0108320.
Gleizal
,
A.
,
Ferreira
,
S.
,
Lavandier
,
B.
,
Simon
,
B.
,
Beziat
,
J. L.
, and
Bera
,
J. C.
, 2010
, “
The Impact of Low Intensity Pulsed Ultrasound on Mouse Skull Bone Osteoblast Cultures
,” Rev. Stomatol. Chir. Maxillo-Fac.
,
111
(5–6
), pp. 280
–285
.10.1016/j.stomax.2009.07.01321.
Carina
,
V.
,
Costa
,
V.
,
Pagani
,
S.
,
De Luca
,
A.
,
Raimondi
,
L.
,
Bellavia
,
D.
,
Setti
,
S.
,
Fini
,
M.
, and
Giavaresi
,
G.
, 2018
, “
Inhibitory Effects of Low Intensity Pulsed Ultrasound on Osteoclastogenesis Induced In Vitro by Breast Cancer Cells
,” J. Exp. Clin. Cancer Res.
,
37
(1
), p. 197
.10.1186/s13046-018-0868-222.
Uddin
,
S. M. Z.
,
Hadjiargyrou
,
M.
,
Cheng
,
J. Q.
,
Zhang
,
S.
,
Hu
,
M. Y.
, and
Qin
,
Y. X.
, 2013
, “
Reversal of the Detrimental Effects of Simulated Microgravity on Human Osteoblasts by Modified Low Intensity Pulsed Ultrasound
,” Ultrasound Med. Biol.
,
39
(5
), pp. 804
–812
.10.1016/j.ultrasmedbio.2012.11.01623.
Jiang
,
W. J.
,
Sun
,
S. X.
,
Xu
,
Z.
, and
Ta
,
D. A.
, 2017
, “
Finite Element Simulation of Local Sound Field Distribution When LIPUS Irradiates Bone Cells
,” Tech. Acoust.
,
36
(6
), pp. 549
–555
(in Chinese).24.
Wang
,
L. P.
,
Hsu
,
H. Y.
,
Li
,
X.
, and
Xian
,
C. J.
, 2016
, “
Effects of Frequency and Acceleration Amplitude on Osteoblast Mechanical Vibration Responses: A Finite Element Study
,” BioMed Res. Int.
,
2016
, pp. 1
–16
.10.1155/2016/273509125.
Kubo
,
T.
,
Fujimori
,
K.
,
Cazier
,
N.
,
Saeki
,
T.
, and
Matsukawa
,
M.
, 2011
, “
Properties of Ultrasonic Waves in Bovine Bone Marrow
,” Ultrasound Med. Biol.
,
37
(11
), pp. 1923
–1929
.10.1016/j.ultrasmedbio.2011.08.00526.
Metzger
,
T. A.
,
Schwaner
,
S. A.
,
LaNeve
,
A. J.
,
Kreipke
,
T. C.
, and
Niebur
,
G. L.
, 2015
, “
Pressure and Shear Stress in Trabecular Bone Marrow During Whole Bone Loading
,” J. Biomech.
,
48
(12
), pp. 3035
–3043
.10.1016/j.jbiomech.2015.07.02827.
Frost
,
H. M.
, 2004
, “
A 2003 Update of Bone Physiology and Wolff's Law for Clinicians
,” Angle Orthodontist
,
74
(1
), pp. 3
–15
.https://pubmed.ncbi.nlm.nih.gov/15038485/28.
Tang
,
L.
,
An
,
S. S.
,
Zhang
,
Z. H.
,
Fan
,
X. S.
,
Guo
,
J. Z.
,
Sun
,
L. J.
, and
Ta
,
D.
, 2021
, “
MSTN Is a Key Mediator for Low-Intensity Pulsed Ultrasound Preventing Bone Loss in Hindlimb-Suspended Rats
,” Bone
,
143
, p. 115610
.10.1016/j.bone.2020.11561029.
Sun
,
S. X.
,
Li
,
L. R.
,
Tang
,
L.
,
Guo
,
J. Z.
, and
Ta
,
D. A.
, 2016
, “
Simulation of the LIPUS-Induced Temperature Field in Bone Using Finite Element Method
,” Proceedings of the 2016 National academic meeting on Acoustics Academic
, Wuhan, China, Oct. 28, pp. 401
–404
(in Chinese).30.
Sun
,
S. X.
,
Sun
,
L. J.
,
Kang
,
Y. T.
,
Tang
,
L.
,
Qin
,
Y. X.
, and
Ta
,
D. A.
, 2020
, “
Therapeutic Effects of Low-Intensity Pulsed Ultrasound on Osteoporosis in Ovariectomized Rats: Intensity-Dependent Study
,” Ultrasound Med. Biol.
,
46
(1
), pp. 108
–121
.10.1016/j.ultrasmedbio.2019.08.02531.
Angle
,
S. R.
,
Sena
,
K.
,
Sumner
,
D. R.
, and
Virdi
,
A. S.
, 2011
, “
Osteogenic Differentiation of Rat Bone Marrow Stromal Cells by Various Intensities of Low-Intensity Pulsed Ultrasound
,” Ultrasonics
,
51
(3
), pp. 281
–288
.10.1016/j.ultras.2010.09.00432.
Uddin
,
S. M. Z.
, and
Qin
,
Y. X.
, 2015
, “
Dynamic Acoustic Radiation Force Retains Bone Structural and Mechanical Integrity in a Functional Disuse Osteopenia Model
,” Bone
,
75
, pp. 8
–17
.10.1016/j.bone.2015.01.02033.
Wang
,
L.
,
Zeng
,
B. F.
, and
Zhang
,
X. L.
, 2005
, “
Experimental Treatment of Osteoporotic Fracture in Rats by Low-Intensity Pulsed Ultrasound
,” Chin. J. Osteoporosis
, 2005(1
), pp. 46
–49
(in Chinese).34.
Costa
,
V.
,
Carina
,
V.
,
Fontana
,
S.
,
De Luca
,
A.
,
Monteleone
,
F.
,
Pagani
,
S.
,
Sartori
,
M.
,
Setti
,
S.
,
Faldini
,
C.
,
Alessandro
,
R.
,
Fini
,
M.
, and
Giavaresi
,
G.
, 2018
, “
Osteogenic Commitment and Differentiation of Human Mesenchymal Stem Cells by Low-Intensity Pulsed Ultrasound Stimulation
,” J. Cell. Physiol.
,
233
(2
), pp. 1558
–1573
.10.1002/jcp.2605835.
Cheng
,
C. C.
,
Chung
,
C. A.
,
Chang
,
C. J.
,
Cheng
,
Y. C.
,
Huang
,
C. J.
,
Chien
,
C. C.
, and
Lin
,
H. T.
, 2022
, “
Hydrostatic Pressure Facilitates Calcium Deposition and Osteogenic Gene Expression in the Osteoblastic Differentiation of Placenta-Derived Multipotent Cells
,” Taiwan. J. Obstet. Gynecol.
,
61
(2
), pp. 270
–276
.10.1016/j.tjog.2022.02.01436.
Nagatomi
,
J.
,
Arulanandam
,
B. R.
,
Metzger
,
D. W.
,
Meunier
,
A.
, and
Bizios
,
R.
, 2003
, “
Cyclic Pressure Affects Osteoblast Functions Pertinent to Osteogenesis
,” Ann. Biomed. Eng.
,
31
(8
), pp. 917
–923
.10.1114/1.159066337.
Wang
,
D. W.
,
Wang
,
H.
, and
Dong
,
F. S.
, 2016
, “
The Research Progress in the Effect of Different Mechanical Stimulation on the Osteoblast
,” Chin. J. Osteoporosis
,
22
(5
), pp. 652
–656
(in Chinese).38.
Mitsui
,
N.
,
Suzuki
,
N.
,
Maeno
,
M.
,
Mayahara
,
K.
,
Yanagisawa
,
M.
,
Otsuka
,
K.
, and
Shimizu
,
N.
, 2005
, “
Optimal Compressive Force Induces Bone Formation Via Increasing Bone Sialoprotein and Prostaglandin E-2 Production Appropriately
,” Life Sci.
,
77
(25
), pp. 3168
–3182
.10.1016/j.lfs.2005.03.03739.
Frost
,
H. M.
, 1987
, “
Bone ‘Mass’ and the ‘Mechanostat’: A Proposal
,” Anat. Rec.
,
219
(1
), pp. 1
–9
.10.1002/ar.1092190104Copyright © 2023 by ASME
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