... were imaged using fluorescence microscopy (FM) and instant polarized light microscopy (IPOL) to visualize the labeled vessels and label-free collagen, respectively. The IPOL images were registered and used to form a stack of FM images from which the vessels were segmented and skeletonized...

... the cell are quantified according to the fluorescence intensities recorded. It is found that the amounts of nanoparticles attached onto the cell membrane and inside the cell both increase with temperature. Based on the experimental results, a model is proposed to describe the cellular uptake dynamic...

... bone diagnostic radiography fluorescence regression analysis in vivo patellar tracking trochlear groove geometry patellofemoral joint fluoroscopy The tracking of the patella within the femoral trochlear groove is controlled via complex interactions between the articular geometry...

... to denaturation when exposed to extremes of p H and temperature. We have utilized intrinsic fluorescence and Fourier transform infrared spectroscopy to determine the structural transitions of encapsulated lysozyme in the range of − 120 ° C < T < 100 ° C . At cryogenic temperatures encapsulated lysozyme did...

... biorheology deformation fluorescence optical microscopy proteins Injury to axons in the white matter of central nervous system (CNS) tissue is the largest contributor to physiological and functional deficits following brain and spinal cord trauma ( 1 2 ). Myelinated axons, myelinating glia...

... ± SD , n = 7 ) for these two material populations, respectively. The linear structures in AFM images were identified by fluorescence microscopy as bundles of f-actin, or stress fibers. After treatment with 4 μ M cytochalasin B, HAECs behaved like a homogeneous linear elastic material with an apparent...

... information from ICG dye angiograms about blood velocity distribution in the choriocapillaris and its feeding blood vessels. ICG dye fluorescence intensity rise and decay curves are constructed for each pixel location in each image of the choriocapillaris in an ICG angiogram. It is shown that at each instant...

...Walter J. Akers; Mark A. Haidekker Blood viscosity changes with many pathologic conditions, but its importance has not been fully investigated because the current methods of measurement are poorly suited for clinical applications. The use of viscosity-sensitive fluorescent molecular rotors...

...W. Akers; M. A. Haidekker Background : Molecular rotors exhibit viscosity-dependent quantum yield, allowing non-mechanical determination of fluid viscosity. We analyzed fluorescence in the presence of viscosity-modulating macromolecules several orders of magnitude larger than the rotor molecule...

... Interferometry (Laser Doppler Velocimetry). Deposition, as assessed qualitatively using fluorescent dye, is related to the position of the laryngeal jet within the trachea. The position of the jet is dependent on the downstream conditions of the model. It is proposed therefore that lung/airway conditions may...

... such branches, not just along the midline. Short-term uptake of rhodamine-labeled albumin, measured using digital imaging fluorescence microscopy of serial sections, was greatest in an approximately triangular region downstream of immature branches, but in mature animals it was greater upstream, particularly...