A PED (precision extrusion deposition)/replica molding process enables scaffold guided tissue engineering of a heterocellular microfluidic device. We investigate two types of cell-laden devices: the first with a 3D microfluidic manifold fully embedded in a PDMS (polydimethylsiloxane) substrate and the second a channel network on the surface of the PDMS substrate for cell printing directly into device channels. Fully embedded networks are leak-resistant with simplified construction methods. Channels exposed to the surface are used as mold to hold bioprinted cell-laden matrix for controlled cell placement throughout the network from inlet to outlet. The result is a 3D cell-laden microfluidic device with improved leak-resistance (up to 2.0 mL/min), pervasive diffusion and control of internal architecture.
Fabrication of Microfluidic Manifold by Precision Extrusion Deposition and Replica Molding for Cell-Laden Device
3141 Chestnut Street,
Philadelphia, PA 19104;
Contributed by the Manufacturing Engineering Division of ASME for publication in the JOURNAL OF MANUFACTURING SCIENCE AND ENGINEERING. Manuscript received March 5, 2015; final manuscript received August 26, 2015; published online October 27, 2015. Assoc. Editor: Yong Huang.
- Views Icon Views
- Share Icon Share
- Search Site
Snyder, J., Rin Son, A., Hamid, Q., and Sun, W. (October 27, 2015). "Fabrication of Microfluidic Manifold by Precision Extrusion Deposition and Replica Molding for Cell-Laden Device." ASME. J. Manuf. Sci. Eng. April 2016; 138(4): 041007. https://doi.org/10.1115/1.4031551
Download citation file: