With today's computing technology, research on soot particles using simulation works has become more preferable as a supplementary to the existing experimental methods. The objective of this study is to investigate the effect of different engine load conditions to in-cylinder soot particles formation. This is to clarify the relationship between soot mass fraction (SMF) and size distribution. The first section of the study is conducted by computational analysis using a detailed kinetics soot model, particulate size mimic (PSM), which is based on the concept of the discrete sectional method. The analysis is carried out within closed-cycle combustion environment which is from the inlet valve closing (IVC) to the exhaust valve opening (EVO). The next section is conducted by experimental work deliberately for validation purpose. The total soot mass obtained from the computational work during EVO is comparable to the calculated value by less than 13% error for all of the experimental cases. The soot size distribution measurement indicates that exhaust out particles are dominantly in the dual-mode size range, <10 nm and 11–30 nm. The relationship between the soot mass and size distribution demonstrates that soot mass fraction does not completely rely on soot size distribution as well as particle size range. In most of the cases, particles with the moderate size range (11–60 nm) hold the highest mass fraction during EVO. On the whole, this paper provides significant information that contributes key knowledge to indicate that soot mass fraction is not entirely dependent on soot size distribution as well as particle size range.